Averaging interaction energies over homologs improves protein fold recognition in gapless threading

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Averaging interaction energies over homologs improves protein fold recognition in gapless threading.

Protein structure prediction is limited by the inaccuracy of the simplified energy functions necessary for efficient sorting over many conformations. It was recently suggested (Finkelstein, Phys Rev Lett 1998;80:4823-4825) that these errors can be reduced by energy averaging over a set of homologous sequences. This conclusion is confirmed in this study by testing protein structure recognition i...

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Protein sequence threading: Averaging over structures.

Multiple sequence alignments are a routine tool in protein fold recognition, but multiple structure alignments are computationally less cooperative. This work describes a method for protein sequence threading and sequence-to-structure alignments that uses multiple aligned structures, the aim being to improve models from protein threading calculations. Sequences are aligned into a field due to c...

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Protein fold recognition by prediction-based threading.

In fold recognition by threading one takes the amino acid sequence of a protein and evaluates how well it fits into one of the known three-dimensional (3D) protein structures. The quality of sequence-structure fit is typically evaluated using inter-residue potentials of mean force or other statistical parameters. Here, we present an alternative approach to evaluating sequence-structure fitness....

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We present a comprehensive analysis of methods for improving the fold recognition rate of the threading approach to protein structure prediction by the utilization of few additional distance constraints. The distance constraints between protein residues may be obtained by experiments such as mass spectrometry or NMR spectroscopy. We applied a post-filtering step with new scoring functions incor...

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Motivation Protein fold recognition when appropriate, evolutionarily-related, structural templates can be identified is often trivial and may even be viewed as a solved problem. However in cases where no homologous structural templates can be detected, fold recognition is a notoriously difficult problem ( Moult et al., 2014 ). Here we present EigenTHREADER, a novel fold recognition method capab...

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ژورنال

عنوان ژورنال: Proteins: Structure, Function, and Genetics

سال: 1999

ISSN: 0887-3585,1097-0134

DOI: 10.1002/(sici)1097-0134(19990515)35:3<353::aid-prot9>3.0.co;2-e